Abstract
Conditions of optical microscopes and ways to use them are directly related to the easiness or difficulty of ICSI procedures. The attached instruction book explains how to use and maintain the microscope in detail. In terms of an optics maker, perusing the instructions are strongly recommend. However, some users sometimes claim that they feel difficulty to understand an instruction. In this paper how to use and maintain the microscopes used in ICSI-for example a stereoscopic microscope, a phase contrast microscope, an inverted microscope fitted with Nomarski differential interference optics and an inverted microscope with Hoffman optics-is plainly explained with a lot of figures.
