Abstract
In this study, Escherichia coli mutant IM303 was used to detect the effect of pulsed magnetic fields through reactive oxygen species. IM303 is a mutant defective in the superoxide dismutases Fe-SOD and Mn-SOD. The strength of the exposed magnetic field was 0.06, 0.1, or 0.13 T. Trains of 10 or 5 pulses of magnetic field at intervals of 0.05 s were applied at 1 Hz. The growth of the mutant was detected by measuring the light absorbance at 600 nm every hour. After six hours of exposure, cell were centrifuged and two-dimensional gel electrophoresis of the proteins in E. coli was performed. Two-dimensional gel electrophoresis data revealed lost protein in the exposed samples of IM303. Their growth rate did not change with exposure. The results of this study indicate that the synthesis of proteins that do not change the cell cycle length has been altered. However, lethal damage to IM303 did not occur.
