Abstract
It is reported that large dose of D-Galactosamine (Gal N) induce some cellular changes in rat liver. The cellular changes are described to be closely similar to that of human viral hepatitis. However, very few works have been carried out to make experimental liver lesion with Gal-N using various strains of mice. The present paper is to evaluate a possibility of using mice as an experimental model of the human viral hepatitis.
C3H/He and dd mice treated with Gal-N showed the marked increase in serum transaminase (GPT, GOT) activity and the large accumulation of inflammatory cells in liver histologically. These changes in mice just reveal a striking similarity to the human viral hepatitis. The responses in sera and liver cells were most remarkable at 24hrs and 48hrs after treated with Gal-N. After 72hrs, the biochemical and histological restitutions to normal stage were observed. Female group showed the responses stronger both in biochemical and histological changes than the male group, and dd mice were more sensitive to Gal-N than C3H/He. From these observations, a possibility was suggested that C3H/He and dd mice treated with Gal-N could be used for experimental model of the human viral hepatitis like disease. Further studies to elucidate the difference in sensitivities against Gal-N under various conditions of strains, sex and age are also necessary.
