Abstract
Using the method of disc electrophoresis on poly-acrylamide gel, single radial immuno diffusion (SRID) and radio-immunoassay (RIA) method, CSF electro-phoretic pattern and immunoglobulins (lgG, IgA, IgM, IgD, IgE) were analyzed in 142 samples from normal control subjects (15) and patients with neurological diseases, including multiple sclerosis (9), myopathies (22), CVD (58), degenerative diseases (18), brain tumors (14) and infectious diseases (6).
The protein fractions of CSF was compared with those of serum in normal human subjects, and it was shown that CSF contains a high proportion of prealbumin and a low proportion of albumin.
CSF disc electrophoresis in M.S. showed a high proportion of γ-globulin and a low proportion of albumin. In myopathy group, albumin proportion was high, but its origin is unknown.
Mean values of normal CSF immunoglobulins were IgG : 1.7 mg/dl, IgA : 0.2 mg/dl, IgE : 12IU/dl. But CSF IgM and IgD were so little that those could not be detected. Mean values of IgG%, IgA%, IgG (c/s) % and IgA (c/s) % in all neurological disorders, were much more than those levels in control group.
In M.S., both IgG (%) and IgG (c/s)% were elevated. It was suggested that the reason of those high levels is the result of elevation of immunoglobulin IgG produced by brain.
In myopathic diseases, levels of IgG% and IgG (c/s) % were also increased. As the origin of the increase, the latter was suspected of the influence of blood-CSF barrier.
Level of IgM (c/s) % was increased in brain tumor, in cerebral infarcts and in meningitis, but levels in those diseases were only traceable except in metastatic tumor.
IgD could not be detected in CSF.
I could not detect any specific relations between control group and neurological disease group with IgE and IgE (c/s) % in CSF.
