Abstract
In order to clarify the relationship between the kinins and endotoxemia caused by intestinal obstruction. An experimental study in the process of internal obstruction was made concerning changes of bradykinin in the blood. After strangulated obstructions were performed to dogs' small intestines, their plasma-kinin in the blood gradually increased and showed a rapid increment at the time of their deths, resulting in the average survival time of 18 hours. This increment of plasma-kinin was inhibited by means of the anti-kinin agent (Trasylol) administered into the vein after the intestinal obstruction was made and it was observed that the survival time was slightly prolonged in a group of dogs administered with 30, 000 U/kg. Furthermore, administration of a non-absorbing antibiotic (fradiomycin) so as to decrease the number of intestine bacteria prior to the intestinal obstruction, the increase of plasma-kinin was clearly inhibited and as a result, their survival times were conspicuously prolonged.
Administration of E. coli 0-26: B-6 (1mg/kg) into the dogs' vein caused an obvious appearance of endotoxin in 5 minutes in the blood. Then the highest level of plasma-kinin was observed 1-3 minutes after endotoxin appeared and after this peak it gradually returned to the normal range. However, in the case of seriously suffered dogs from shock, the highest level did not return to the normal range and consequently they died in 30 minutes. A similar pattern was observed in the changes of hemodynamics of hepatocirculation in both cases of administering endotoxin and administering bradykinin to dogs.
As regards the protective effect of Trasylol against the lethal toxicity of endotoxin was clearly observed in an experiment using a group of mice.
Seen from these results, it can be concluded that plasma-kinin should be one of the most important chemical mediators concerned with endotoxemia in the process of intestinal obstruction.
