Abstract
It has been reported that procedures for purifying of the B-cell for pancreas islet's cells can be made more efficient by applying the affinity chromatography technique.
The immunoabsorbent of affinity chromatography was prepared by using agarose beads treated with an antibovine insulin antibody. The islet's cells were obtained from the collagenase-isolated rat's islets by Dispase-treatment, and then, they were incubated with the immunoabsorbent described above.
About 30 percent of the cells were absorbed. The cells were identified as B-cells by aldehyde fuchsin staining and by the enzyme labeled antibody technique.
The results show that treatment with a high concentration of glucose in the incubationmedium always produces increases of insulin-release from the cells and their O2 consumption. The procedures are recommended as useful in studies on the insulin-release mechanism of the B-cell.
