Immunohistochemical localization of Na⁺, Kt⁺-ATPase and calmodulin in rat myocardium

Abstract

The immunohistochemicloacl alizatioonf Na⁺, K⁺-ATPase and calmodulin was investigated in rat heart muscle using antiserum against the catalytic subunit of the human kidney Na⁺, K⁺-ATPase and bovine testis calmodulinat the electronm icroscopilce ve. l Immunostaininfgo r both Na⁺, K⁺-ATPase and calmodulin was noted on the plasmam embranea nd plasmalemmal vesicleos f the vasculare ndotheliuman dc ardiacm usclecellsC. almoduliwna sa lsod etectedin t he cytoplasmT. hesei mmunohistochemicloacl alizationws ereevaluatedb y immunobloatn alyse. s Such localizatioonf Na⁺, K⁺-ATPase and calmodulinsu ggeststh at they mayp laya n important rolei n the regulation of Na⁺-Ca⁺⁺ exchange across the cell membrane.