Abstract
1. Microbiological assay of pantethine was investigated, using Lactobacillus bulgaricus B1. Although the bacillus responds to pantothenic acid with about 1/25 sensibility, the pantethine level may be determined by substructing the pantothenic acid value obtained by Lactobacillus fermenti ATCC 9338.
2. Pantethine was never recognized in the urine of normal adults.
3. Pantethine was neither eliminated in the urine when pantothenic acid had been given intramusculary in healthy adults. Urinary elimination of pantethine was very low even when pantethine had been loaded. The sum of pantethine and pantothenic acid in the urine was only about one half of the case of pantothenic acid loading.
4. It is assumed that pantethine has higher reabsorbability in uriniferous tubules and higher tissue affinity than pantothenic acid.
