Abstract
Gas chromatography for the identification of vitamin D2, 5, 6-trans vitamin D2, isocalciferol (isovitamin D2), isotachysterol2, tachysterol2, lumisterol2 and ergosterol was investigated, applying the following conditions: apparatus, a Shimazu GC-1B Gas Chromatograph with hydrogen flame ionization detector; column, a U-shaped stainless steel tube (0.4×75cm) packed with silianized 60- to 80-mesh Gas-Chrom W coated with 1.5% SE-30; column temperature, 220°; vaporizer temperature, 250° to 260°; detector temperature, 220°; nitrogen gas flow, 90 to 120ml/min. Lumisterol2 from thermal cyclization products of vitamin D2 (pyro- and isopyrocalciferol), formed during the gas chromatography, 5, 6-trans vitamin D2 from isocalciferol and isotachysterol2 from tachysterol2 were not separated. Therefore, the identification of these compounds was impossible by applying gas chromatography alone.
