Abstract
A microbiological assay procedure for pantethine in the absence of pantothenic acid has been presented, based upon the growth response of L. bulgaricus B-1 strain.
1. The optimum concentrations of Tween 80 and enzymatic digest of casein for the growth of the strain were 50mg per 100ml of the basal medium.
2. The addition of L-glutamic acid and L-tyrosine promoted the growth rate of the strain and the addition of L-tyrosine could especially reduce the incubation time from 40 to 20 hours.
3. From the above result an appropriate basal medium as described in Table 2 was devised.
4. The recovery of Pantethine added to two pharmaceutical preparations was 95-98 per cent when tested by the devised method.
