Abstract
De novo formation of N5-formyl-5, 6, 7, 8-tetrahydropteroyl-glutamic acid and rhizopterin, N10-formylpteroic acid, by Rhizopus oligosporus in a synthetic medium free of folate was observed by the method of bioautography. It was shown that both compounds converted to the expected acids by deformylation with alkaline treatment.
These folate compounds were also identified by comparison of their fluoro spectra and absorption spectra with those of authentic samples.
