Abstract
In addition to our previous findings on GLC and mass spectroscopic determination of vitamins D, extensive studies on the effect of interfering substances have presented a new simple GLC determination method of vitamin D2 in the presence of vitamins A and E. Thus, by employing TLC before applying a sample for GLC evaluation, vitamin E and its related compounds which interfere the GLC determination are eliminated completely and the greater part of vitamin A is also removed in the form of anhydrovitamin A simultaneously. Exhaustive conversion of vitamin A into its anhydro derivative and removal of unreacted vitamin A from vitamin D are not necessary. The proposed method is a simple, inexpensive, accurate, and time-saving quantification method of vitamin D2. Main advantages and limitation of the method are discussed in detail.
