Abstract
Pyridoxine and pyridoxine glucoside are transported into rabbit erythrocytes in vitro. The amounts of vitamin B6 compounds transported into the erythrocytes were bioassayed with Saccharomyces carlsbergensis 4228 ATCC 9080 after hydrolyzing them to free forms of vitamin B6 by autoclaving for 210min in 1/12 N sulfuric acid. The transport of pyridoxine and pyridoxine glucoside was affected by incubation time, incubation temperature and concentration of pyridoxine and pyridoxine glucoside added. The transport of pyridoxine glucoside iincreased with the coexistense of ATP or glucose. The transport of pyridoxine and pyridoxine glucoside decreased by the addition of sodium fluoride or monoodoacetate. The binding of pyridoxine and pyridoxine glucoside by ghost cells was not found. Small amounts of transported pyridoxine and pyridoxine glucoside were leaked out by washings, indicating that large parts of them were retained in the erythrocytes.
