Abstract
1. In acidic and neutral media, DBT shows a reduction wave, which is considered to be due to the reduction of the carbonyl group.
2. Only in a more acidic medium than pH 5, DAT shows the reduction wave. The behavior of DAT is therefore similar to that of thiamine.
3. Both DAT and DBT show an anodic oxidation wave in an alkaline medium, and after the treatment with sodium hydroxide both compounds show a typical protein wave. These facts mean that DAT and DBT behave similarly to thiamine, since the sulfhydryl group is freed in an alkaline medium.
4. The polarographic determination of DAT and DBT respectively can be carried out at pH 1.0 and 0.2N sodium hydroxide solution.
