STUDIES ON THIAMINE-DECOMPOSING THERMOSTABLE FACTORS

III. ON A REACTION PRODUCT PRODUCED BY THE REACTION BETWEEN THIAMINE AND RUTIN

Abstract

The reaction of thiamine with rutin was investigated and the reaction product was isolated in a crystalline form. This product is as active as thiamine to love-birds. It is reduced by cysteine to thiamine in vitro. By hydrolysis 2 methyl 4 amino 5 amino-methylpyrimidine is formed, which was isolated and identified. Following administration to rats, a marked rise in urinary thiamine excretion was observed. From these facts it was presumed that the reaction product is a new derivative belonging to a disulfide type of thiamine. It was tentatively called “rutinothiamine”. Its possible structure was discussed.