Abstract
1. FHD was synthesized from FAD by deamination with nitrous acid and the product was first isolated as barium salt. The yield and purity were, however, unsatisfactory by this method. Therefore, the product was extracted with cresol, followed by transfer in aqueous phase, and by subjecting to column chromatography, FHD was obtained in high purity in satisfactory yield.
2. Various physical and chemical properties of FHD were examined and its components were confirmed by hydrolysis.
3. Physiological activity of FHD was tested using D-amino acid oxidase but no remarkable activity was observed.
4. FHD formation from FAD by the action of adenosine deaminase obtained from Takadiastase could be proved both spectrophotometrically and chromatographically, but this method seems to be unadequate for preparation because of partial decomposition of FHD.
5. The spectroscopic characteristic of FHD was determined and compared with that of a mixture of FMN and inosinic acid.
