STUDIES ON THE DISULFIDE FORM OF THIAMINE

III. REFORMATION OF DISULFIDE AFTER REDUCTION OF DISULFIDE-THIAMINE BY ACTIVE THIOL COMPOUNDS IN AN ALKALINE MEDIUM

Abstract

1. After TPD or B₁SSB₁ is reduced to free thiamine by cysteine or GSH, the re-formation of the disulfide-thiamine occurs in an aerobic and weakly alkaline medium at some mole ratios and concentrations.
2. B₁SSB₁ reacts similarily to TPD.
3. The reducing action of GSH upon the disulfide-thiamine is observed to be much stronger than that of cysteine.