Abstract
1. An extract of E. coli catalyzed the successive transglycosidation between sucrose and B2, synthesizing various B2-compounds of oligosaccharides, containing either glucose or fructose.
2. Tentative structures of these B2-compounds were characterized by paper chromatographic isolations, followed by partial acid hydrolysis.
3. B2-fructoside synthesized by E. coli, was isolated in a crystalline form and its chemical properties were found to be identical with those of B2-fructoside synthesized by Asp. oryzae.
4. It was found that glucose, α-methylglucoside and α-glucose-1-phosphate inhibited the transglucosidation, while fructose preferably inhibited the transfructosidation.
5. Raffinose was capable of replacing sucrose as a fructosyl donor, but fructose was hardly utilized.
