Abstract
1. In the absorption spectrum of the purified clearing factor two absorption maxima were recognized, i.e., 270-280 and 410-420mμ.
2. Lipolytic activity reduced by dialysis was recovered by pyridoxal phosphate, but not by heparin. It is probable that one component necessary for the activity of the lipolytic system is pyridoxal phosphate.
3. Optimal pH of the enzyme activity was 8.0.
4. The enzyme was shown to exist in albumin fraction electrophoretically.
5. The enzyme was inhibited by protamine sulfate, quinine sulfate, pCMB and heparin, but not by glutathione.
