THE JOURNAL OF VITAMINOLOGY
Online ISSN : 2185-2553
Print ISSN : 0022-5398
COLORIMETRIC ESTIMATION OF VITAMIN A AND β-CAROTENE WITH ANTIMONY PENTACHLORIDE
TOSHIRO MURATAJUNKO NAGASHIMA
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1960 Volume 6 Issue 2 Pages 158-162

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Abstract
Vitamin A and β-carotene form with SbCl5 color substances having an absorption maximum at 620mμ, at which wavelength the colorimetric estimation can be easily and rapidly carried out.
Sufficient color for estimation is obtained by adding 0.01ml of SbCl5-chloroform solution containing 468mg of SbCl5 per ml to 3.5ml of sample chloroform solution.
Staining the glass by the SbOCl formed in the procedure was insignificant with the use of SbCl5.
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© THE VITAMIN SOCIETY OF JAPAN
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