Abstract
1. FAD was adsorbed on several commercial active carbon powder, with the capacity of from 10 to 15% of their weight. It was eluted with basic solvents more easily than with acidic solvents. Of the solvents tested, the most adequate were pyridine-water (1:1) and lutidine-water (2:3). The FAD adsorbed on active carbon was recovered in a yield of 80% or more after five successive elutions with lutidine-water (2:3) using 10 fold volume of the carbon weight. No purifying effect was observed in the above process.
2. FAD with neary 90% purity was obtained from the crude FAD preparation through formation of barium and silver salts and removing the metal ions. This purifying effect was principally due to the silver salt process and the yield was about 70% including the succeeding process.
