DECREASE OF APOENZYME ACTIVITIES IN VITAMIN-DEFICIENT ANIMALS

II. DECREASE OF OXIDATIVE PHOSPHORYLATION IN VITAMIN B₁-DEFICIENT RATS

Abstract

1. In the present paper it was studied whether thiamine activated the oxidative phosphorylation of various substrates in the presence of the washed liver homogenate of thiamine-deficient rats.
TABLE II Oxidative Phosphorylation of Pyruvic Acid^a
TABLE III Qo₂(N) for the Phosphorylation of Various Substrates in the Presence of Washed Liver Homogenate
TABLE IV P/O Ratios for Various Substrates
2. To the reaction system pyruvic acid, α-ketoglutarate, L-proline, L-glutamic acid, and L-valine were added. The decrease of P/O ratio was seen in thiamine-deficient homogenate, but no such phenomenon occurred in thiaminepositive control animals.