Abstract
1. V compound was produced non-enzymatically from G compound.
2. The conversion reaction of G compound into V compound proceeds enzymatically in the presence of the enzyme preparation obtained from the spinach.
3. The enzymatic formation of V compound from G compound requires the presence of molecular oxygen, and inhibited completely under anaerobic condition.
4. The enzymatic reaction to form riboflavin from G compound proceeds without molecular oxygen.
5. The stoichiometry of the enzymatic synthesis of riboflavin was established by the reaction under “anaerobic condition”.
6. Formation of V compound was inhibited by KCN and KF.
