1963 Volume 9 Issue 4 Pages 313-316
The effects of various nitrogen and carbon sources alone or in combination on the growth of Propionibacterium shermanii were examind for the purpose of enhancing the production of DBCC. It was found that the addition of bonito extract to the Bernhauer medium from which Casamino acid and Tryptose had been omitted, resulted in a 20% increase in bacterial growth. When OH-B12 was added to the improved medium, about 80% of the active substance was found to have been transfered to the cells and moreover, the active substance was found to be mostly 5, 6-dimethylbezimidazolylcobamide coenzyme.