Abstract
Organic content and availability as nutrients were investigated for bacteria in compacted bentonite and asphalt material, and possibilities of bacteria migration in compacted bentonite were examined. Total organic carbon and humic substances in the bentonite (Kunigel V1) were estimated more than 0.30 to 0.35 wt%, 0.5 to 10 ppm, respectively. Bacteria in groundwater were propagated in the presence of bentonite as much as twofold greater than in the absence of it. A straight asphalt was separated into 4 fractions by solvent extraction and by chromatographic technique. Growth of some Pseudomonas strain was observed in some fractions. Finally, the experimental system was shown for a better understanding of bacterial migration in compacted bentonite.