Effects of calcium hydroxide on expression of genes associated with hard tissue resorption in human osteoblastic cells stimulated by rhIL-1β

Abstract

We previously studied the effect of calcium hydroxide on the LPS-induced expression of hard tissue resorption-related genes in human osteoblastic cells (Saos-2), and reported that LPS-induced inflammatory cytokine expression and that the constant expression of RANKL was inhibited by calcium hydroxide. The objective of this study was to evaluate the effect of calcium hydroxide on the IL-1-induced expression of hard tissue resorption-related genes as a part of evaluation of the efficacy of calcium hydroxide for inflammatory hard tissue resorption. We used real-time reverse transcriptase-polymerase chain reaction to evaluate the effect of calcium hydroxide on the expression of genes of TNF-α, IL-6, OPG and RANKL synthesized by rhIL-1β-stimulated human osteoblast-like cells. The data were compared employing Student's t-test (n=6) with p<0.05 considered significant. Calcium hydroxide inhibited TNF-α and IL-6 gene expression in rhIL-1β-stimulated Saos-2, inhibited the enhancement of RANKL gene expression, and inhibited constant RANKL gene expression. Calcium hydroxide appeared to both resolve non-bacterial inflammation and inhibit hard tissue resorption.