Synthetic calcium particles as a new scaffold have porosity and extended-release properties

Abstract

When our artificially synthesized peptide (ASPep) with a scaffold that provides extended release was cutaneously placed in rats, it induced hard tissue formation. We focused on synthetic calcium particles (SCPs) made of calcium phosphate-coated calcium carbonate to develop a better scaffold. We performed three different experiments to investigate the properties of SCPs. We observed the surface of SCPs by scanning electron microscope (SEM); following saturation in albumin the amount of the albumin released from the SCPs was measured in vitro after they were saturated in albumin; and we observed the biological reaction to SCPs saturated in ASPep using a monoclonal antibody to ASPep (MoAb Pep) in vivo. The SEM findings indicated that the surface of the SCPs was porous. In an in vitro study, the concentration of albumin gradually increased following elution from the SCPs. In another in vivo study, the immuno reaction to the MoAb ASPep was localized in the central region of the SCP at 14 days, wherereas that reaction in the outer region of the SCP had disappeared. It is suggested that the ASPep in the SCPs was eluted from the surface layer of the SCPs by day 7, while that in the central region remained longer and was more slowly released. (J Osaka Dent Univ 2021; 55: 53-58)