Radiosensitivity of an isolated human malignant fibrous histiocytoma cell line

Shoji KAWASAKI; Akifumi MIZUTA; Naoko KASHITANI; Masahiro KURODA; Yoshio HIRAKI; Kaname AONO; Jiro SATO; Tetsuro OGINO; Takashi NAKANISHI

doi:10.4044/joma1947.101.1-2_1

Shoji KAWASAKI, Akifumi MIZUTA, Naoko KASHITANI, Masahiro KURODA, Yoshio HIRAKI, Kaname AONO, Jiro SATO, Tetsuro OGINO, Takashi NAKANISHI

Author information

Shoji KAWASAKI
School of Health Sciences, Okayama University
Akifumi MIZUTA
Department of Radiology, Okayama University Medical School
Naoko KASHITANI
Department of Radiology, Okayama University Medical School
Masahiro KURODA
Department of Radiology, Okayama University Medical School
Yoshio HIRAKI
Department of Radiology, Okayama University Medical School
Kaname AONO
Department of Radiology, Okayama University Medical School
Jiro SATO
Division of Pathology, Cancer Institute, Okayama University Medical School
Tetsuro OGINO
Division of Pathology, Kagawa Medical School Hospital
Takashi NAKANISHI
Department of Radiology, Yamaguchi University Medical School

Keywords: Tissue culture, Malignant fibrous histeocytoma, Radiosensitivity, Flow cytometry

JOURNAL FREE ACCESS

1989 Volume 101 Issue 1-2 Pages 1-6

DOI https://doi.org/10.4044/joma1947.101.1-2_1

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Abstract

A human cell line (YU-137) isolated from a bone of malignant fibrous histiocytoma in bone was evaluated using immunofluorescent staining methods. Cell cycle fractions were studied by flow cytometry. The magnitude of G₂+M fraction in a cell cycle of an exponentially growing population of YU-137 was higher than that observed in HeLa S3 cells. The doubling time of YU-137 cells was 3.2 days. The radiosensitivity of this cell line was compared with HeLa S3 and HMV-1 cells from dose response curves against X-irradiation. The parameters of radiosensitivity of YU-137 against X-irradiation were n=2.0, D₀=1.05 Gy and D₀=0.73 Gy. From these data, the radiosensitivity of YU-137 was discussed.

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