Abstract
A modified injection replica SEM method was introduced. Thorough injection of a resin mixture (monomeric methacrylate containing 1% benzoyl peroxide and 1% N, N-dimethylaniline) prior to the microwave treatment prepares good and fine blood vascular casts or replicas of brain, hypophysis, pineal body, thyroid gland and other organs. These casts sufficiently withstood ionbombardment and were useful for scanning electron microscopy. In this casting, preliminary perfusion fixation prior to the resin injection was always necessary because the mixture was toxic to the vascular walls and destroyed them.
