STUDIES ON JAPANESE B ENCEPHALITIS VIRUS (2)

ON THE SEROLOGICAL REACTIONS BY THE LYOPHYLIZED VIRUS STRAINS AND THE HEMAGGLUTINATION REACTION STORED CHICK ERYTHROCYTES

Abstract

1) Making a comparative study of the virus strains which were preserved by lyophilization and the strains serially passaged in the mice brains of several ten generation, the following results were observed.
The formcr strains completely retook their toxity passaging in the mice brains of serial 23 generations. Any difference could not be demonstrated between the both strains in the point of the infective titer to mice. Inoculating the both strains to mice respectively, the titer of the antigen for complement fixation reaction showed the approximately same value, while in the titer of hemagglutinin lyophilized strains showed higher value.
2) For the purpose of preserving the reactivity of chick erythrocytes in the hemagglutination reaction, the following medical solution mixed with chick blood. In case of 2% Na-oxalate, the reactivity was preserved best, 2.5% Na-citrate and 0.1% heparin next, and modified Alsever's solution worst. And yet in the last case, the reactivity was demonstrated after two or three weeks.