Studies on the Tissue Culture of Rabbit's Bone Marrow

Part II. The Influence of Temperature

Abstract

The influences of temperature upon the tissue culture of rabbit's bone marrow were investigated.
The effects of various temperatures from 3° to 37°C were tested by Carrel's bottle method, in which a specially designed apparatus was used in this experiment. The lowest temperature was 12°C at which the proliferation of cultivated tissue was scarcely ascertained. The proliferation was nearly proportional to the temperature-rise in the range of from 12° to 37°C.
Another test was carried out by the slide culture method from 33°C to 41°C. At 41°C proliferation was not observed any more, and the maximal proliferation was obtained at 39°C. But this proliferation at 39°C was not the normal one but somewhat irritated or abnormal one. Thus, for the ordinary observation, cultivation at 37°C is thought to be more suitable.
The migration rate of pseudo-eosinophilic leukocyte showed an increase in proportion to the ascent of temperature. But the great migration rates at high temperatures did not last long and fell very soon. The maximal migration rate was observed at 43°C. The increased migration rate lasted for at least 6 hours at these high temperatures. Up to 55°C the leukocytes did not lose their migratory power.
The cultured tissue could be kept alive for 16 days at 3°C but the proliferating power of the tissue decreased day by day.