The Influence of Cancer Tissue Extract on the Pancreas of Experimental Animals

Part I. The Influence of the Cancer Tissue Extract on the External Pancreatic Secretion

Abstract

The cancer tissue extract was made from surgically removed stomach cancer. A pancreatic fistula was made on dog and the change of the content of the various ferments in the pancreatic juice was studied after daily intravenous administration of 10% cancer tissue extract 1 cc/kg.
The following results were obtained;
1) Diastase was markedly decreased. The decrease began on the 1st day of the experiment, and the lowest value occurred in 2nd or 3rd day. This lowest value was maintained as long as the injection of the cancer tissue extract were continued. However, when injection of cancer tissue extract was discontinued, diastase values were gradually increased.
2) Trypsin also began to decrease on the 1st or 2nd day after injection of the cancer tissue extract. The lowest value occurred on the 2nd or 3rd day of the experiment. However, compared to diastase, this decrease was not marked.
3) There was found no marked change regarding the content of lipase.
4) In the control group, in which the extract of normal stomach tissue was used as injection material, there were found no change in the ferments of pancreatic juice.
The nucleic acid in the pancreatic tissue was studied histochemically by the Pironin-Methylgreen stain using rabbits which were given the cancer tissue extract 1 cc/kg daily intravenously.
The following results were obtained;
1) The amount of RNA was decreased moderately after daily injection of the cancer tissue extract for 2 weeks and marked decrease was found after daily injection for 4 weeks.
2) No significant changes were observed in DNA and KES.
It is believed that the function of external pancreatic secretion is definitely inhibited by the injection of the cancer tissue extract.