Abstract
With the purpose to reveal the relationship between hemoglobin (Hb) synthesis and lipid synthesis, the author studied the intra-cellular appearance of Hb and lipids cytochemically. The absorption of heme at 4, 060 Å in the cells appears as early as in the stage of basophilic erythroblast, both in the cytoplasm and the nucleus.
In the nucleus it appears in the interchromatin spaces and diffusely in cytoplasm.
With the progress of maturation of the cells heme-absorption increases in its intensity, and in the stages of orthchromatic erythroblast it reaches to the level of that of reticulocyte. The absorption in the nucleus generally disappears with the pycnosis of the nucleus and the absorption reaches the maximum in matured red cells.
The globin synthesis observed by Giemsa staining proves that this protein appears in the same stage and site as heme. The peroxydase reaction which will be given by Hb also shows the same tendency as in the case of heme and globin.
These results show that the site of the heme absorption seems to indicate the site of Hb synthesis. By observing erythroid cells in each maturation stage, stained with Sudan Black B, a quantity of lipids has been detected always in the site where Hb is detected, showing that the Hb synthesis is always accompanied by lipid synthesis.
