Abstract
By means of the precipitation reaction, the author studied the antigenic significance of various fractions extracted from cholera vibro and, in addition to this, the variation of the cellular composition present between the newly isolated strain and the previously isolated and preserved ones. The results are briefly summarzed as follows.
1) Of all the extrats from cholera vibrio, Boivin's was concluded to be the only one which was of use as the precipitinogen, and Julianelle's substance, crude nucleoprotein and others had not only low antigenic titers but also little type specificity.
2) Boivin's substance, which is significant as the antigen, shows a specific precipitation reaction; manifestations of antibody titers in the range of low and high dilutions had an aspect of reaction with two sorts of antigen. Designated the former as X antigen and the latter as Y antigen, X antigen reacted upon the homologous serum and Y antigen upon the heterologous one, and antigenic titers seemed to be higher upon the homologous serum and lower upon the heterologous one.
3) From manifestation of precipitation reaction, some difference was proved to be present between the newly isolated and preserved ones.
