Abstract
On the frozen-dried tissues prepared by using the apparatus designed by SENO a scrutiny has been carried out for the freezing, embedding and the fixation.
Observations on those embedded in paraffin reveald that different results can be obtained by the different treatment followed; the treatment with peteroleum-ether for the deparaffinization with the simultaneous fixation of tissues often causes the destruction of the cell structure, prefixation of the sections by floating on 40% formol or 80% alcohol prior to the deparaffinization gives a good result. However, the tissues embedded in paraffin often yield some crevices at sectioning. Embedding in methacrylate in vacuum, proved to be the best by which the tissues can be preserved in a good condition, sectioned and stained without removing the methacrylate. If needed, the methacrylate can be removed by amyl-acetate or acetone after the fixation by the method just described. Thin sections for the electron microscopy is also available in the material embedded in methacrylate.
