Immunofluorescent Studies on the Tumor Induced by Adenovirus Type 12

2. On the Cross-Antigenicity between Tumor Antigen and Normal Hamster Tissue

Abstract

The author previously reported the high contents of tumor antigens both in the fractions of microsome and mitochondria and the localization of them on the tumor cells induced by Adenovirus type 12.
In this report, the cross-reactivity between tumor antigens and normal hamster tissues was studied by direct method of immunofluorescent technic using anti-microsome antibody conjugated and by indirect method using sera from tumor bearing hamsters.
1. Direct method
Specific immunofluorescence was demonstrated in the normal brain tissue, restrictedly at the ependymal layer, the meninx and the surroundings of almost all of small blood vessels.
No specific fluorescence was observed in any other organs excepting perichondrium.
2. Indirect method
Sandwitch method using sera from tumor bearing hamsters revealed also the specific fluorescence granularly in the ependymal layer cells and diffusely in the meninx.
These peculiar sites showing positive fluorescence in the brain corresponded with the predilecting parts of the intracranial tumor of newborn hamsters injected with adenovirus type 12.
Furthermore the specific fluorescence was more distinct in embryos or newborn than in young or adult hamsters.