Studies on ⁹⁰YCl₃ Colloid for Clinical Use

Part I. Purification of ⁹⁰YCl₃ Colloid by Milking Method and the Observation of Electrone Microscopy

Abstract

⁹⁰Y is a high-energy radioisotope and emite β-ray with 2.6 days half life. ⁹⁰YCl₃ colloid has been used for malignant tumor therapeutically, as ¹⁹⁸Au colloid.
In the present experiment, the purification of ⁹⁰Y, and the detection of the contaminated ⁹⁰Sr was studied. Amberlite XE-100 column was suitable for this prupose. The colloidal size of ⁹⁰YCl₃ was transformed by pH, and the features of ⁹⁰YCl₃ colloids were observed on electrone microscope.
1) ⁹⁰Y and ⁹⁰Sr mixture adsorbed on Amberlite XE-100 column (100-200 mesh, 2.5 column volume), and eluted by 0.5N HCl. ⁹⁰Sr is eluted out at 10-60ml, and ⁹⁰Y is eluted out from 170ml.
2) A mixture of 20mC of citrate-⁹⁰Y complex and 1μC of ⁹⁰Sr fixed on this column, and then eluted by 0.5N HCl. One drop of eluate at 10ml was detectable by GM counter. The detectable lower limit of contaminated ⁹⁰Sr is 0.1μC by re-counting on GM counter 24hrs after the elution.
3) Amberlite XE-100 column is suitable for the second column on the milking system, for the purpose of separating the contaminated ⁹⁰Sr and ⁹⁰Y. This procedure is complete in 1hr process.
4) ⁹⁰YCl₃ colloid is aggregated and formed clamp in high pH solution, Electrone microscopic observations showed that the colloidal size was 40-50mμ at pH 5, and 20mμ at pH 7.