Phosphatidic Acid Metabolism in DAB Induced Hepatoma

Part 2. The distributions of ³²P freed from phosphatidic acid to main phosphalipids in DAB-feeding rat liver

Abstract

Male Donryu rats were fed with 3 methyl diaminoazobenzen (DAB). The liver was homogenized and incubated with ³²P-phosphatidic acid at 20, 40, 60, 80 and 100th day. The distributions of ³²P freed from phosphatidic acid by phosphatidic acid phosphatase were studied on lysolecithin, sphingomyelin, phosphatidylcholin and phosphatidylethanolamin. The distribution for lysolecithin and sphingomyelin were no differences between normal and DAB-feeding rat liver. The distribution for phosphatidylcholin was elevated at 80th day of DAB-feeding rat liver than the normal one, and the distribution for phosphatidyl ethanolamin was bighn in DAB-feeding rat liver than the normal one at 20th day, but on the controry, it was changed at 40 and 60th day, and increased in normal than in DAB-feeding rat liver.