Studies on the Measurement of Human Blood Catalase Activity and the Screening Method of Hypocatalasemia by Spectrophotometry

Part 2. Measurement of human blood catalase activity by spectrophotometry with a temperature controlled cell chamber and autorecorder

Abstract

For the spectrophotometry a Hitachi Perkin-Elmer 139 type spectrophotometer with a constant temperature cell chamber and autorecorder was used.
The measurements were conducted under the following conditions (final concentration of H₂O₂ 0.0125 Mol in 0.01 Mol phosphate buffer, pH 6.8), which showed the identical conditions with a exception of H₂O₂ being 2.5 times the concentration employed in the titration method.
Both assays were carried out at 37°C.
The results by ultraviolet spectrophotometric method (UV method) were compared with those obtained by titration; the values obtained by UV method were about 90% of those by titration, and proved that each values showed a good correlation. Therefore this method will sufficiently serve for the measurement of human blood catalase activity or for the screening of hypocatalasemia.