Disruption of the Nuclei of Rat Liver and AH-130 Cells by Nitrogen Pressure Bomb and the Effect on RNA Polymerase Activity

Abstract

Nitrogen pressure bomb was used for the disruption of nuclei. The effects of disruption were checked by electron microscopy and estimtion of released RNA-polymerase. Disruption of rat liver nuclei was incomplete in the presence of 3 mM Mg⁺⁺ and complete in the presence of 0.02mM Mg⁺⁺, in which nuclear membrane and nucleoli were completely disrupted.
For releasing RNA polymerase from AH-130 nuclei, disruption of nuclei was done by sonication in the presenec of 0.3M ammonium sulphate. RNA-polymerase activity was lost makedly by sonication. In the same condition AH-130 nuclei was disrupted by nitrogen pressure bomb, in which RNA polymerase activity was not lost and it was recovered in the supernatant fractino with the yield of 90%.