Abstract
Changes in gastric mucosal prostaglandin (PG) E1 in rats with acute gastric mucosal lesions caused by water immersion stress or by brain damage were studied. Rats with brain damage were prepared by injecting cyanoacrylate into the brain. PGs were extracted from gastric mucosae, and PGE1 was separated by silicic acid column chromatography and converted into PGB by alkaline treatment. PGE1 was measured by radioimmunoassay using a double antibody method with anti-PGB1 antibody.
In normal rats, values of PGE1 in the gastric mucosa were 249.3+64.3 pg/mg protein (mean+standard deviation). In the rats which underwent water immersion stress, PGE1 values were significantly elevated (p<0.01) above the normal values 2, 3, and 6 hours after the treatment. In the rats with brain damage, PGE1 values did not change significantly for 3 hours after the brain damage. However, 6 hours after the treatment, PGE1 values were significantly elevated (p<0.01) above the normal values.
In the rat which underwent water immersion stress, the elevation of PGE1 was inhibited by intraperitoneal administration of atropine sulfate, diazepam, hexamethonium bromide or 6-hydroxy dopamine (6-OHDA). In contrast, in the rats with brain damage, the elevation of PGE1 was significantly suppressed only by hexamethonium bromide and 6-OHDA which suppress the sympathetic nerve.
