Abstract
The mucoid, rough and non-mucoid types organisms ofStreptococcus mutansFIL strain were respectively cultured in the liquid media supplemented individually with three kinds of sugars, and growth phases of respective organisms were studied turbidometrically and by viable bacterial count. Under the experimental conditions used in this study, however, no visible effect of sugar types added was observed in the growth pattern of all these organisms. It was of interest, however, that only sucrose among other sugars showed an effect to accerelate the doubling time of bacterial count with three types of organisms. The peculiar agglutination reaction of growing organisms occurring in the sucrose media and causing difficulty in accurate counting of bacteria has been avoided in this study by addition of 0.5 mg/ml of a low molecular dextran preparation 1.04×104 to the sucrose-added liquid media, and this procedure made it easy to obtain reproducible growth curves in such studies.
