The histological and histochemical studies on hypocalcified dentin occurred in the incisor and the abnormal bone formed in alveolar bone of rat given SrCO³

Abstract

The histological and histochemical studies on hypocalcified dentin which occurred in incisor, and abnormal bone which formed in the alveolar bone, of a rat given strontium carbonate were made.
The hypocalcified dentin and abnormal alveolar bone hardly reacted to hematoxylin, toluidine blue (pH.2.5), and alcian blue (pH.1.0) stains. The hypocalcified dentin reacted intensely to eosin and van Gieson stains, and showed a strong PAS reaction, compared with those in normal dentin. However, as for the PAS reaction in hypocalcified dentin, further investigation will be required.
The abnormal alveolar bone reacted to eosin and van Gieson stains. The degree of PAS reaction in the abnormal alveolar bone was lower than that in normal alveolar bone.
These observations, suggest that the occurrence of hypocalcified dentin and abnormal alveolar bone depends upon the disturbance of mucopolysaccharide and collagen metabolisms
in the calcification process of hard tissues.