Studies on the salivary metabolic factor (VII)

Acetone preparation of metabolic factor in human salivary supernatant

Abstract

In order to separate the metabolic factors (MF) in human centrifugated salivary supernatant, acetone was added to the salivary supernatant to make final concentrations of 10, 30, 40, 50, 70, 90%.
These were divided into acetone soluble and insoluble fractions.
The test medium (total volume, 3 ml) containing acetone treated fractions, salivary sediment and 0.02 M glucose solution, was incubated at 37°C for 2 hours by the tube-culture method under aerobiccondition.
The glucose content was measured by the glucose oxidase method.
Most of the MF was detected in the soluble fractions at acetone concentrations of 10, 30, 40% and showed ninhydrin positive reaction.
The acetone separation method is considered to be most effective in the isolation of MF.