A histochemical study of periodontal disease
A fundamental study on the localization of prostaglandin (PG)
1985 Volume 27 Issue 4 Pages 1132-1145
Details
1985 Volume 27 Issue 4 Pages 1132-1145
In order to investigative the localization of PG in gingival tissue by the direct immuno-fluorescein antibody technique, a fundamental study was undertaken both on the preparation of the antigen and on the separation, specificity, staining of the antibody.
1) The quantity of PG used to bind PG to carrier-protein in this experiment was as little as half the amount of PG which had so far been used. Molar ratio of PG-protein products was approxi-mately 10 moles of PG per one mole of carrier-protein.
2) 10ml antiserum was purified by an affinity column chromatography coupling of PGE to AHSepharose. As the result, 8mg PG-specific antibody was obtained. 3) The cross-reactivity of the antibody described above 16 per cent against PGF2α, 13 per cent against PGB2, and less than 5 per cent against A1, A2, E1, F1α, respectively.
4) Even 0.2 per cent protein solution was able to be labelled with fluorescein isothiocyanate (FITC).
5) It seemed, that the PG staining in rat uterus, as well as the specificity of the antibody examined by the present author was better than those obtained by Van Orden.
6) PG was localized in cytoplasms like the macrophage observed beneath the gingival crest and the oral gingival epithelia in periodontal disease. But PG was not localized in the area in which many lymphocytes were observed to infiltrate beneath the oral epithelium.
