Construction of a bovine gingival cDNA library

Abstract

We have constructed a bovine gingival cDNA library consisting of approximately 9×10⁶λgt10 clones, from 20g of gingival tissue powder which was prepared by freezing and pulverization in liquid nitrogen. One hundred clones or more were isolated randomly from the library, and their insert cDNA's were analyzed by polymerase chain reaction (PCR). Agarose gel electrophoresis of the PCR-amplified products revealed that most of the analyzed clones harbored insert cDNA's of lengths ranging from 300 to 1100 in base-pairs.
Our library will be useful for exploring the structure of various genes expressed in gingiva, and mechanisms which may control the expression of the genes in health and disease.