Abstract
Hydrolysis of soy phosphatidylcholine (S-PC) to lysophosphatidylcholine by using phospholipase A2 was conducted under various conditions without stirring. The degree of hydrolysis was determined based on the amounts of phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) by high performance liquid chromatography (HPLC). HPLC was simple, rapid and reproducible for quantitative analysis of PC and LPC in S-PC hydrolysate.
The hydrolysis of S-PC depended on reaction temperature, reaction time, CaCl2 concentration, and buffer.
S-PC was more easily hydrolyzed by Tris-hydrochloric acid than by H3BO3-KCl-NaOH, NaH2PO4-Na2HPO4 or Na2HPO4-citric acid as buffer.
Optimum S-PC hydrolysis conditions were as follows : Tris-hydrochloric acid buffer; pH 8, CaCl2; 0.3 wt%/S-PC, 60°C, S-PC/aqueous medium; 1/2 (g/mL), phospholipase A2; 10IU.
