Expression of ras-P21 and ras Gene Alteration in Pleomorphic Adenomas

Seijiro OKUTSUI; Akio TAKEDA; Tsubura SUZUKI; Yoshitaka NAKAJIMA; Junichi SATO; Katsuyuki SUDA; Masakatsu FUKUDA; Tsutomu USAMI; Takao HIMIYA; Kaoru KUSAMA; Itsuro KUDO; Itaru MORO

doi:10.2334/josnusd1959.35.200

Expression of ras-P21 and ras Gene Alteration in Pleomorphic Adenomas

Seijiro OKUTSUI, Akio TAKEDA, Tsubura SUZUKI, Yoshitaka NAKAJIMA, Junichi SATO, Katsuyuki SUDA, Masakatsu FUKUDA, Tsutomu USAMI, Takao HIMIYA, Kaoru KUSAMA, Itsuro KUDO, Itaru MORO

Author information

Seijiro OKUTSUI
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
Akio TAKEDA
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
Tsubura SUZUKI
Department of Oral Surgery, Saitama Medical Center, Saitama Medical School
Yoshitaka NAKAJIMA
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
Junichi SATO
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
Katsuyuki SUDA
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
Masakatsu FUKUDA
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
Tsutomu USAMI
Department of Pathology, Nihon University School of Dentistry
Takao HIMIYA
Department of Pathology, Nihon University School of Dentistry
Kaoru KUSAMA
Department of Pathology, Nihon University School of Dentistry
Itsuro KUDO
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
Itaru MORO
Department of Pathology, Nihon University School of Dentistry

Keywords: polymerase chain reaction, temperature-gradient gel electrophoresis, pleomorphic adenoma, ras oncogene, ras-P21

JOURNAL FREE ACCESS

1993 Volume 35 Issue 3 Pages 200-203

DOI https://doi.org/10.2334/josnusd1959.35.200

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Abstract

The expression of ras-P21 protein and gene alteration of the K-ras gene were examined in 12 cases of pleomorphic adenoma of the salivary gland. The tumor cells in all 12 cases were strongly stained for ras-P21 protein by the indirect immunoperoxidase method, suggesting the enhanced expression of ras-P21 protein in pleomorphic adenomas. Analysis of the PCR products for the K-ras gene by temperature-gradient gel electrophoresis (TGGE) revealed that gene alterations such as point mutations occurred in 4 out of 12 pleomorphic adenoma tissues examined.

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