Plasma Adiponectin Level and Peripheral Blood Leukocyte Adiponectin Receptor Expression in Obese Dogs as Obese Indicators

Abstract

The aim of this study is to determine and compare the effects of obesity on plasma adiponectin levels and peripheral blood leukocyte (PBL) adiponectin receptor 1 and 2 (ADIPO-R1 and -R2) expression in the obese dogs. Biochemical examination of blood including plasma enzymes, hormones and cytokines were measured concurrently. Expression levels of ADIPO-R1 and -R2 mRNA were determined by quantitative RTPCR. As results, obese dogs demonstrated a significant increase in insulin, FFA, LDH levels and a significant reduction in circulating adiponectin level compared to the control dogs. In genetically analysis, relative ADIPO-R1 and ADIPO-R2 mRNA expression levels on PBL compared to control PBL were also significantly higher in the obese dogs. Adiponectin receptor (ADIPO-R1 and ADIPO-R2) mRNA expression levels in the obese dog PBL were generally up-regulated compared to those of the control animals. Recently there are various observations for adiponectin concentration and adiponectin receptors mRNA expression levels in obesity. Further studies in obese dogs are needed for adiponectin and adiponectin receptors in order to be useful indicators.