2018 Volume 21 Issue 1 Pages 7-12
In humans, the rate of acetaminophen（APAP）absorption reflects the rate of gastric emptying. However, few studies have reported the use of the APAP method for evaluating gastric emptying in dogs. The purpose of the current study was to investigate whether a commercial APAP detection kit can measure serum APAP in healthy dogs. First, we evaluated the validity of this kit, measuring canine serum levels after the administration of APAP. Analytical evaluation of serum APAP concentrations indicated acceptable precision,with intra-assay coefficients of variation（CV）not exceeding the commonly accepted 10％limit. However,the inter-assay CV for serum APAP concentrations was 19.4％. A linear relationship was demonstrated with all dilutions tested, up to a 16-fold dilution. Next, to determine the detectable APAP dose using this kit, six healthy dogs were administered the experimental diet alone（0 mg（APAP）/kg（body weight））and the experimental diet with APAP added, at doses of 10 mg/kg and 20 mg/kg. A significant increase was observed in serum APAP concentrations at a dose at 20 mg/kg, compared with doses of 0 mg/kg and 10 mg/kg. In conclusion, an APAP detection kit is able to measure serum APAP concentrations in dogs and may be applicable to determine gastric emptying. Furthermore, the minimum detectable APAP dose in dogs is approximately 20 mg/kg.
